Precedent and Justice

Precedent is the cornerstone of common law method. It is the core mechanism by which the common law reaches just outcomes. Through creation and application of precedent, common law seeks to produce justice. The appellate courts\u27 practice of issuing unpublished, non-precedential opinions has generated considerable discussion about the value of precedent, but that debate has centered on pragmatic and formalistic values. This essay argues that the practice of issuing non-precedential opinions does more than offend constitutional dictates and present pragmatic problems to the appellate system; abandoning precedent undermines justice itself. Issuance of the vast majority of decisions as nonprecedential tears the justice-seeking mechanism of precedent from the heart of our common law system

Chromogranin A in the pancreatic islet

Chromogranin A (CGA) is the major soluble protein within secretory vesicles of chromaffin cells. A polyclonal antiserum was raised against bovine CGA and characterized in two-dimensional immunoblots. Cellular and subcellular distribution of CGA in bovine pancreatic islet was investigated by immunocytochemistry. At the light microscopic level, CGA-like immunoreactivity was found in the same cells that react with antibodies against insulin, glucagon, and somatostatin. A minority of cells containing pancreatic polypeptide also showed faint immunostaining. At the ultrastructural level (protein A-gold technique), CGA-like immunoreactivity was confined exclusively to the secretory vesicles. Whereas the hormones were localized mainly in the central part of the secretory vesicles, CGA was present predominantly in the periphery. These findings indicate that a CGA-like protein is a regular constituent of the matrix of secretory vesicles in pancreatic endocrine cells

Characterization of hormone and protein release from alpha-toxin- permeabilized chromaffin cells in primary culture

Addition of Staphylococcus aureus alpha-toxin to adult bovine chromaffin cells maintained in primary culture causes permeabilization of cell membrane as shown by the release of intracellular 86Rb+. The alpha-toxin does not provoke a spontaneous release of either catecholamines or chromogranin A, a protein marker of the secretory granule, showing the integrity of the secretory vesicle membrane. However the addition of micromolar free Ca2+ concentration induced the co-release of noradrenaline and chromogranin A. In alpha-toxin-treated cells, the released chromogranin A could not be sedimented and lactate dehydrogenase was still associated within cells, which provides direct evidence that secretory product is liberated by exocytosis. By contrast, permeabilization of cells with digitonin caused a Ca2+- dependent but also a Ca2+-independent release of secretory product, a dramatic loss of lactate dehydrogenase, as well as release of secretory product in a sedimentable form. Ca2+-dependent exocytosis from alpha- toxin-permeabilized cells required Mg2+-ATP and did not occur in the presence of other nucleotides. Thus alpha-toxin is a convenient tool to permeabilize chromaffin cells, and has the advantage of keeping intracellular structures, specifically the exocytotic machinery, intact

Dynamics of coupled vortices in layered magnetic nanodots

The spin dynamics are calculated for a model system consisting of magnetically soft, layered nanomagnets, in which two ferromagnetic (F) cylindrical dots, each with a magnetic vortex ground state, are separated by a non-magnetic spacer (N). This permits a study of the effects of interlayer magnetostatic interactions on the vortex dynamics. The system was explored by applying the equations of motion for the vortex core positions. The restoring force was calculated taking into account the magnetostatic interactions assuming a realistic surface charge free spin distribution. For tri-layer F/N/F dots with opposite chiralities and the same core polarizations (lowest energy state), two eigenmodes are predicted analytically and confirmed via micromagnetic simulations. One mode is in the sub-GHz range for submicron dot diameters and corresponds to quasi-circular rotation of the cores about the dot center. A second mode is in the MHz range corresponding to a small amplitude rotation of the mean core position. The eigenfrequencies depend strongly on the geometrical parameters of the system, suggesting that magnetostatic effects play a dominant role in determining the vortex dynamics.Comment: One PDF file including text and 4 figure